Thapsigargin inhibits voltage-activated calcium channels in adrenal glomerulosa cells

Thapsigargin inhibits voltage-activated calcium channels in adrenal glomerulosa cells.

Thapsigargin, an inhibitor of the microsomal Ca2+ pumps, has been extensively used to study the intracellular Ca2+ pool participating in the generation of the agonist-induced Ca2+ signal in various cell types. A dual effect of this agent was observed in bovine adrenal zona glomerulosa cells. At nanomolar concentrations, thapsigargin stimulated a sustained Ca2+ influx, probably resulting from Ca...

Subunit of Voltage-activated Calcium Channels*

Voltage-activated Ca channels play a major role in many fundamental physiological processes including neurotransmission, muscle contraction, intracellular signaling, hormone secretion, and development. Therefore, an understanding of the structure and regulation of Ca channels is critical for the comprehension of these physiological phenomena. Five types of high voltage-activated Ca channels (na...

Gamma subunit of voltage-activated calcium channels.

Pore stability and gating in voltage-activated calcium channels.

Calcium channel family members activate at different membrane potentials, which enables tissue specific calcium entry. Pore mutations affecting this voltage dependence are associated with channelopathies. In this review we analyze the link between voltage sensitivity and corresponding kinetic phenotypes of calcium channel activation. Systematic changes in hydrophobicity in the lower third of S6...

Large Depolarization Induces Long Openings of Calcium Channels in Adrenal Chromaffin Cells Voltage-Dependent

Single Ca*+-channel currents in bovine adrenal chromaffin ceils were studied with the patch-clamp technique using Ba2+ as the charge carrier. Depolarizing pulses to voltages less than + 10 mV from holding voltage of -60 mV elicited short openings with a mean life time of less than 1 msec. Depolarization to more positive voltages elicited longer openings with a mean life time of about 3 msec in ...